ylxQ [2019-06-07 17:23:50]

similar to ribosomal protein, L7AE family, binds K-turns in RNA switches

locus: BSU16620

pI: 10.17

mw: 10.92 kDa

protein length: 100 aa

gene length: 303 bp

function: unknown

product: unknown

essential: no

synonyms: ymxC,rpmXA,ktuQ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.5|Ribosomal protein/ based on similarity]

Gene

Coordinates: 1,733,687 1,733,989

The protein

Catalyzed reaction/ biological activity

• binds K-turns in [SW|RNA switch]es as the occur in the [protein|search|L-box], the [protein|search|S-box] and the [protein|search|T-box] [Pubmed|22355167]

Protein family

• [SW|ribosomal protein] L7Ae family (according to Swiss-Prot)

Structure

• [PDB|3V7Q] [Pubmed|22355167]

Expression and Regulation

Operons

• genes: [gene|9F6C447900410BF64409E5C41A11C76968DADAFA|ylxS]-[gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA]-[gene|F4097349A563503468A2A14F062AEAC532C7917A|ylxR]-[gene|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA]-[gene|378B77412D0DEA220A97DEC246410509D9B74D46|infB]-[gene|24835CE6CD2E1F183DB9121F29A63101718B5DF9|ylxP]-[gene|B3119A40393A288EF34FC3E9839B6E40AC6A6EA0|rbfA]
  description: [Pubmed|8491709]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8491709], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|15805528,26577401], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, in [regulon|stringent response|stringent response]
  • [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|NusA]: attenuation, [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|NusA] stimulates termination [Reference|http://www.nature.com/articles/nmicrobiol20157], in [regulon|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|NusA regulon]

  regulation

  • autoregulation [SW|NusA] [http://www.nature.com/articles/nmicrobiol20157 reference]
  • induced by glucose [pubmed|30355672]

Biological materials

Mutant

• BKE16620 ([gene|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGGAAACCATTCCATTCCAG, downstream forward: _UP4_AATAAGCTGATCAGCTTGCTCG
• BKK16620 ([gene|7BFA65857F69855DBB3BB64D93B4ED2F09DC705B|rplGA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGGAAACCATTCCATTCCAG, downstream forward: _UP4_AATAAGCTGATCAGCTTGCTCG

References

• 11948165,8491709,22355167,29280348,26522935